anti panck  (MedChemExpress)

Journal: Journal of Nanobiotechnology

Article Title: Fucoidan-decorated metal-zoledronic acid nanocomplexes suppress tumor metastasis by inducing ferroptotic cell death and enhancing cancer immunotherapy

doi: 10.1186/s12951-025-03473-0

Figure Lengend Snippet: FTZ@Fu inhibits tumor metastasis and attenuates the immunosuppressive TME. ( A ) Hematoxylin and eosin (H&E) staining of spontaneous lung metastasis from orthotopically implanted 4T1-LM cells with different MNCs formulations treatments. Bar = 100 μm. Quantification of metastatic tumor colonies is shown. ( B ) 3D images showing tumor (PanCK) and vasculature (CD31) in mice lung. Enlarged pictures indicate micro- (blue area) and macrometastasis (pink area). ( C ) Representative images of the expressions of Ki67, γH2AX, IFN-γ, and granzyme b in nuclei (DAPI) in tumor tissues. Quantitative data are shown in the right panel. Bar = 100 μm. ( D ) Representative images of Treg and CD8 T-cell infiltration in tumor tissues by MNC treatment. Quantitative data are shown in the right panel. Bar = 100 μm. * p < 0.05; ** p < 0.01; and *** p < 0.001

Article Snippet: Each lung tissue sample was labeled with 40 μL of mouse anti-PanCK monoclonal antibody (Cell Signaling, #4545), followed by 40 μL of goat anti-CD31 polyclonal antibody (R&D Systems, AF3628).

Techniques: Staining